Up-regulation of IL-6 and TNF-α induced by SARS-coronavirus spike protein in murine macrophages via NF-κB pathway
- 作者：Wei Wang ; Linbai Ye ; Li Ye ; Baozong Li ; Bo Gao ; Yingchun Zeng ; Lingbao Kong ; Xiaonan Fang ; Hong Zheng ; Zhenghui Wu ; Yinglong She
- 关键词：SARS-coronavirus ; Spike protein ; IL-6 ; TNF-α ; NF-κ ; B ; Macrophages
- 刊名：Virus Research
- 出版年：2007
- 期刊代码：92_01681702
- 类别：imm
- 出版时间：September 2007
- 卷：128
- 期：1-2
- 页码：1-8
- 文件大小：542 K
摘要
The clinical picture of severe acute respiratory syndrome (SARS) is characterized by an over-exuberant immune response with lung lymphomononuclear cells infilteration and proliferation that may account for tissue damage more than the direct effect of viral replication. To understand how cells response in the early stage of virus–host cell interaction, in this study, a purified recombinant S protein was studied for stimulating murine macrophages (RAW264.7) to produce proinflammatory cytokines (IL-6 and TNF-α) and chemokine IL-8. We found that direct induction of IL-6 and TNF-α release in the supernatant in a dose-, time-dependent manner and highly spike protein-specific, but no induction of IL-8 was detected. Further experiments showed that IL-6 and TNF-α production were dependent on NF-κB, which was activated through I-κBα degradation. These results suggest that SARS-CoV spike protein may play an important role in the pathogenesis of SARS, especially in inflammation and high fever.