Tim-2 up-regulation and galectin-9-Tim-3 pathway activation in Th2-biased response in Schistosoma japonicum infection in mice

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• 作者：Yao Qi^a ; ¹&#xA0 ; [Author Vitae] ; Xiao-rong Song^a ; ^d ; ¹&#xA0 ; [Author Vitae] ; Ji-long Shen^a ; ^{shenjilong53@126.com} ; ^{xiaorong9028@126.com}&#xA0 ; [Author Vitae] ; Yuan-hong Xu^a ; ^c&#xA0 ; [Author Vitae] ; Qian Shen^a ; ^b&#xA0 ; [Author Vitae] ; Qing-li Luo^a&#xA0 ; [Author Vitae] ; Zheng-rong Zhong^a&#xA0 ; [Author Vitae] ; Wei Wang^a&#xA0 ; [Author Vitae] ; De-yong Chu^a&#xA0 ; [Author Vitae] ; Wen-jian Song^e&#xA0 ; [Author Vitae]
• 关键词：Tim ; T cell immunoglobulin domain and mucin domain ; S. japonicum ; Schistosome japonicum ; Gal-9 ; galectin-9 ; SEA ; soluble egg antigen ; Th cells ; T helper cells ; IFN-纬 ; interferon gamma ; IL-4 ; interleukin-4
• 刊名：Immunology Letters
• 出版年：2012
• 期刊代码：115_01652478
• 类别：med
• 出版时间：30 May, 2012
• 卷：144
• 期：1-2
• 页码：60-66
• 文件大小：1149 K

摘要

T cell immunoglobulin domain and mucin domain (Tim) family, a new gene that expresses on the surface of T cells, plays a critical role in regulation of T cells response. Previous data have shown that Tim-3 expressed on Th1 cells promotes itself apoptosis. Tim-2 is preferentially up-regulated during Th2 differentiation and functions as a potent costimulatory molecule for T-cell immunity. The present study aims to learn whether Tims are responsible for Th2-biased response evoked by Schistosoma japonicum infection. The expressions of Tim-2 and Tim-3 in spleen lymphocytes from S. japonicum-infected mice were examined, and the possible role of galectin-9-Tim-3 pathway in Th2-biased response triggered by schistosome infection was discussed. Our results showed that Tim-2 mRNAs were up-regulated in the spleen of schistosome-infected mice, which coincided with elevated IL-4 gene expression. Administration of galectin-9 significantly induced apoptosis of na茂ve spleen lymphocytes with down-regulation IFN-纬expression in vitro. Additionally, Tim-3-Fc fusion protein notably enhanced Th1 cells and decreased Th2 cells in vitro. Thus, we concluded that pro-apoptotic effects on Th1 population through galectin-9-Tim-3 pathway and the up-regulation of Tim-2 on Th2 cells might be critical to Th2-biased response of host with schistosomiasis japonica.