Corrigendum to “Immune responses to recombinant Mycobacterium smegmatis expressing fused core protein and preS1 peptide of hepatitis B virus in mice” [J. Virol. Methods 141 (2007) 41–48]
- 作者：Xingbin Hu ; Wen Yin ; Qiaohong Yue ; Xueqing Xu ; Sanhua Wei ; Yingfeng Lei ; Xin Lü ; Jing Yang ; Mingquan Su ; Xiaoke Hao ; Zhikai Xu
- 关键词：Fluorescent probes ; Nucleolar targeting ; Hop stunt viroid ; Peptide ; Nucleolus
- 刊名：Journal of Virological Methods
- 出版年：2007
- 期刊代码：180_01660934
- 类别：med
- 出版时间：September 2007
- 卷：144
- 期：1-2
- 页码：172
- 文件大小：72 K
摘要
Viroids are small, single-stranded, circular, non-coding pathogenic RNAs. Hop stunt viroid (HSVd) is characterized by possesses rod-like structure and replicate in the host nuclei. Green fluorescent protein (GFP) fusions with transit sequences or entire proteins can be used for deliberate labelling of particular cell compartments. Different GFP-fusions have been obtained to selectively illuminate different organelles and membranes in many cell types. However, as far as we know, examples for established efficient markers for nucleoli are scarce. In this work, a viroid-RNA was made translatable by inserting an ATG at position 1 and fused to the GFP. The results showed that the resultant fusion can be used as an efficient “in vivo” nucleolar marker in “real time” cellular observations. Thus, this construct can be a very useful tool to study processes related with nucleolus functions.