The dynamic expression of Mash1 in the hippocampal subgranular zone after fimbria-fornix transection

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• 作者：Chuanming Dong^a ; Heyan Zhao^a ; Weiwei Chen^b ; Lei Wang^a ; Lei Zhang^a ; Xinhua Zhang^a ; Jinhong Shi^a ; Haoming Li^a ; Guohua Jin^a ; ^{jguohua@ntu.edu.cn}
• 关键词：NSCs ; neural stem cells ; DG ; dentate gyrus ; SVZ ; subventricular zone ; SGZ ; subgranular zone ; CNS ; central nervous system ; BrdU ; 5-bromo-2-deoxy-uridine ; FF ; fimbria-fornix ; bHLH ; basic helix-loop-helix ; NGF ; nerve growth factor-1 ; BDNF ; brain-derived neur
• 刊名：Neuroscience Letters
• 出版年：2012
• 期刊代码：52_03043940
• 类别：neu
• 出版时间：27 June, 2012
• 卷：520
• 期：1
• 页码：26-31
• 文件大小：744 K

摘要

Mash1, a member of the basic helix-loop-helix (bHLH) transcription factor family, has previously been considered essential for neuronal differentiation and specification in the nervous system. In this study, we investigated the expression of Mash1 in the hippocampus after fimbria-fornix (FF) transection. Western blot showed that protein of Mash1 increased significantly and peaked at day 7 after FF transection. Immunofluorescence indicated that after FF transection, more newborn cells differentiated into Mash1 positive cells in the deafferented side than that in the normal side, and we investigated that in the neurogenic area, subgranular zone (SGZ), a part of Mash1 positive cells were NeuN positive, and more Mash1/NeuN double positive neurons were identified in the deafferented side than that in the normal side. Additionally, the number of Mash1/NeuN double positive neurons in SGZ increased significantly and peaked at day 7 after FF transection. In vitro, immunofluorescence revealed that extracts of the deafferented hippocampus promoted neuronal differentiation to a greater extent than extracts from normal hippocampus. Deafferented extracts also enhanced Mash1 expression in MAP-2 positive neurons. This study concludes that after FF transection, Mash1 expression in the deafferented hippocampus increased and might play an important role in inducing local progenitors to differentiate into neurons.