Study on the retention equation in hydrophilic interaction liquid chromatography

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• 作者：Gaowa Jin ; Zhimou Guo ; Feifang Zhang ; Xingya Xue ; Yu Jin ; Xinmiao Liang
• 关键词：Hydrophilic interaction liquid chromatography ; Retention equation ; Stationary phases ; Selectivity ; Nucleoside
• 刊名：Talanta
• 出版年：2008
• 期刊代码：106_00399140
• 类别：ch
• 出版时间：30 July 2008
• 卷：76
• 期：3
• 页码：522-527
• 文件大小：612 K

摘要

Hydrophilic interaction liquid chromatography (HILIC) is an effective technique for separating polar compounds. But its retention equation has not been studied systematically yet. In this study, an appropriate retention equation was established by using eight nucleosides as model analytes and by comparing four retention models on six different HILIC columns. As a result, retention equation In k = a + b × In C_B + c × C_B could be quantitatively described the retention factors with good accuracy in HILIC mode. Based on this equation, the retention times of eight nucleosides under the conditions by other mobile phase can be predicted on each column. All of the predicted relative errors of retention times were smaller than 5%. The established retention model was also successfully applied to predict retention times of the real Traditional Chinese Medicine—Carthaus tinctorius L. sample.