Chloride intracellular channel 1 is an important factor in the lymphatic metastasis of hepatocarcinoma

详细信息	查看全文 | 推荐本文 |

• 作者：Rong-Kuan Li^a ; Jun Zhang^b ; Yu-Hong Zhang^c ; Miao-Ling Li^b ; Mei Wang^b ; Jian-Wu Tang^b ; ^{jwtang_53@yahoo.cn}
• 关键词：CLIC1 ; RNA interference ; Lymphatic metastasis ; Hepatocarcinoma
• 刊名：Biomedicine and Pharmacotherapy
• 出版年：2012
• 期刊代码：36_07533322
• 类别：med
• 出版时间：April, 2012
• 卷：66
• 期：3
• 页码：167-172
• 文件大小：772 K

摘要

We have previously demonstrated that chloride intracellular channel 1 (CLIC1) is involved in the lymphatic metastasis of tumors. In this study, a self-designed shRNA sequence of mouse CLIC1 gene was synthesized and inserted into a pGPU6/GFP/Neo plasmid, then stably transfected into mouse hepatic carcinoma cell line Hca-F cells to down-regulate the expression of CLIC1 gene. The levels of expression of CLIC1 mRNA and protein were detected by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot (WB) analysis, respectively. The down-regulation of CLIC1 enhanced proliferative activity, increased the ratio of G2/M and decreased percentage of apoptosis. In addition, the capability of migration and invasion decreased significantly. The results indicate that CLIC1 is a critical factor in the development of lymphatic metastasis.