Effective immobilization of Ru(bpy)₃²⁺ by functional composite phosphomolybdic acid anion on an electrode surface for solid-state electrochemiluminescene to sensitive determination of NADH

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• 作者：Yali Li^a ; ^b ; Xiurong Yang^b ; ^{xryang@ciac.jl.cn} ; Fan Yang^b ; Yingping Wang^a ; ^d ; Peihua Zheng^c ; Xiaoxu Liu^c
• 关键词：Electrocatalytic oxidation ; Hybrid nanocomposites ; NADH ; Polyoxometalates ; Self-assembly ; Solid-state electrochemiluminescence
• 刊名：Electrochimica Acta
• 出版年：2012
• 期刊代码：30_00134686
• 类别：ch
• 出版时间：1 April, 2012
• 卷：66
• 期：Complete
• 页码：188-192
• 文件大小：605 K

摘要

Phosphomolybdic acid anion ([PMo₁₂O₄₀]^{3鈭?/sup>) was used for the immobilization of ruthenium(II) tris(bipyridine) (Ru(bpy)₃2+) on an electrode surface to yield a sensitive solid-state electrogenerated chemiluminescence (ECL) sensor. [PMo₁₂O₄₀]3鈭?/sup> anion in the prepared sensor had catalytic ability to the NADH oxidation. The ECL signal of the Ru(bpy)₃2+/[PMo₁₂O₄₀]3鈭?/sup> film was about 3-fold enhancement than that for the Ru(bpy)₃2+/Nafion film to NADH determination. The resulting ECL sensor exhibited a wide linear range from 2.5 脳 10鈭? to 5.0 脳 10鈭? M (R = 0.99) with the detection limit of 1.67 脳 10鈭? M (S/N = 3). In addition, it had good reproducibility and excellent long-term stability, and the relative average deviation was 0.77%of ECL intensity-time curve under continuous potential scanning for 21 cycles; after being used in two weeks, the sensor was able to keep over 90%activity toward 25 渭M NADH. Fabrication of the ECL sensor by this method is simple and easy. Such superior properties will promote the application of polyoxometalates in fabricating sensors for using in electroanalytical and biochemical analysis.}