An advantageous method utilizing new homogenizing device BioMasher and a sensitive ELISA to detect bovine spongiform encephalopathy accurately in brain tissue
- 作者：Takuji Yamamoto ; Yuko Ushiki ; Susumu Hara ; William W. Hall ; Hiroe Tsukagoshi-Nagai ; Takashi Yokoyama ; Yuichi Tagawa ; Tetsutaro Sata ; Yoshio Yamakawa ; Noriaki Kinoshita ; Fjio Tamura ; Shunji Hattori ; Sh
- 关键词：BSE ; ELISA ; Screening kit
- 刊名：Journal of Virological Methods
- 出版年：2008
- 期刊代码：180_01660934
- 类别：med
- 出版时间：May 2008
- 卷：149
- 期：2
- 页码：316-325
- 文件大小：1174 K
摘要
A new screening method was developed to detect bovine spongiform encephalopathy (BSE). This method is advantageous because it has a simpler and safer protocol than commercial kits. A new device was developed for this method; it was named the BioMasher, to homogenize brain tissue by passing it through a porous rigid polypropylene filter. In this system, a purification step was eliminated in the sample preparation. Thus, the time needed for sample pretreatment is substantially shortened, and the risk of infection during sample processing is effectively reduced. Monoclonal antibodies to prion protein were created and used to construct a sensitive sandwich enzyme-linked immunosorbent assay system. The sensitivity of this assay kit using frozen BSE-positive brain is comparable or more sensitive than commercial kits. Moreover, the detection sensitivity for deteriorated samples, which were kept at 37 °C for 1 day, is 10- to 30-fold more sensitive than a commercial kit.