Preparation, structure and anticoagulant activity of a low molecular weight fraction produced by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum

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• 作者：Hongyan Li^a ; Wenjun Mao^a ; ^{wenjunmqd@hotmail.com} ; Yujiao Hou^a ; Yan Gao^b ; Xiaohui Qi^a ; Chunqi Zhao^a ; Yin Chen^a ; Yanli Chen^a ; Na Li^a ; Chunyan Wang^a
• 关键词：Sulfated rhamnan ; Depolymerization ; Structure ; Anticoagulant activity ; Heparin cofactor II
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：June, 2012
• 卷：114
• 期：Complete
• 页码：414-418
• 文件大小：426 K

摘要

A low molecular weight fraction, designated LMWP, was prepared by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum and purified by anion-exchange and gel-permeation chromatography. Chemical and spectroscopic analyses showed that LMWP was mainly composed of rhamnose, and its molecular weight was about 33.6 kDa. The backbone of LMWP consists of 1,3-linked 伪-l-rhamnose units with partially sulfate groups at the C-2 position. Approximately 25%of 1,3-linked 伪-l-rhamnose units is substituted at C-2 by sulfated or non-sulfated 1,3-linked 伪-l-rhamnose and 1,2-linked 伪-l-rhamnose units. LMWP effectively prolonged clotting time as evaluated by the activated partial thromboplastin time assay and was a potent thrombin inhibitor mediated by heparin cofactor II. The investigation demonstrated that LMWP is a novel sulfated polysaccharide with anticoagulant activity.