Clinical evaluation and mitochondrial DNA sequence analysis in two Chinese families with aminoglycoside-induced and non-syndromic hearing loss
- 作者：Lidong Zhao ; Qiuju Wang ; Yaping Qian ; Ronghua Li ; Juayng Cao ; Laura Christine Hart ; Suoqiang Zhai ; Dongyi Han ; Wie-Yen Young and Min-Xin Guan
- 关键词：Mitochondrial 12S rRNA ; Mutation ; Deafness ; Aminoglycoside ototoxicity ; Nuclear modifier genes ; mtDNA ; Chinese ; Haplotype
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2005
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：2005
- 卷：336
- 期：3
- 页码：967-973
- 文件大小：181 K
摘要
We report here the clinical, genetic, and molecular characterization of two Chinese pedigrees with aminoglycoside-induced and non-syndromic hearing impairment. Clinical evaluation revealed the variable phenotype of hearing impairment including audiometric configuration in these subjects. Penetrances of hearing loss in BJ105 and BJ106 pedigrees are 67%and 33%, respectively. In particular, three of 10 affected matrilineal relatives of BJ105 pedigree had aminoglycoside-induced hearing loss, while seven affected matrilineal relatives in BJ105 pedigree and six affected matrilineal relatives in BJ106 pedigree did not have a history of exposure to aminoglycosides. Sequence analysis of the complete mitochondrial genomes in these pedigrees showed the identical homoplasmic A1555G mutation and distinct sets of mtDNA variants belonging to haplogroups F3 and M7b. These variants showed no evolutionary conservation, implying that mitochondrial haplotype may not play a significant role in the phenotypic expression of the A1555G mutation in these Chinese pedigrees. However, aminoglycosides and nuclear backgrounds appear to be major modifier factors for the phenotypic manifestation of the A1555G mutation in these Chinese families.