- 作者：Hideo Kitamura ; Yasushi Matsuzaki ; Kazuyuki Kimura ; Hajime Nakano ; Tadaatsu Imaizumi ; Kei Satoh ; Katsumi Hanada
- 刊名：Journal of Dermatological Science
- 出版年：2007
- 期刊代码：153_09231811
- 类别：med
- 出版时间：February 2007
- 卷：45
- 期：2
- 页码：127-134
- 文件大小：1077 K
Summary
BackgroundRetinoic acid-inducible gene-I (RIG-I) is a member of the DExH/D box family proteins and designated as a putative RNA helicase, which plays various roles in gene expression and cellular functions in response to a variety of RNA viruses.Objective
The present study was designed to investigate the effects of interferon (IFN)-γ and tumor necrosis factor (TNF)-α on RIG-I expression in human keratinocytes, and the expression of RIG-I in skin lesions of psoriasis vulgaris, in which IFN-γ and TNF-α are considered to be involved in its pathogenesis.
Methods
The mRNA and protein expression of RIG-I was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Immunohistochemical staining of RIG-I was examined on psoriatic skin section.
Results
The levels of RIG-I mRNA and protein were upregulated in HaCaT keratinocytes in the presence of IFN-γ or TNF-α. Immunohistochemically, RIG-I was detected in the cytoplasm of the spinous and basal layers of psoriatic skin.
Conclusion
Our results suggest that RIG-I might operate not only as a RNA helicase but also as a mediator of the cytokine network in the inflammatory skin diseases, such as psoriasis vulgaris.