An inhibitory function of WW domain-containing host proteins in RNA virus replication

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• 作者：Jun Qin^a ; ^b ; ¹ ; Daniel Barajas^b ; ¹ ; Peter D. Nagy^b ; ^{pdnagy2@uky.edu}
• 关键词：WW domain ; Host&ndash ; virus interaction ; Antiviral factor ; Tomato bushy stunt virus ; FHV ; NoV ; Replication ; Viral replicase ; Yeast ; Rsp5 ; E3 ubiquitin ligase
• 刊名：Virology
• 出版年：2012
• 期刊代码：108_00426822
• 类别：imm
• 出版时间：10 May, 2012
• 卷：426
• 期：2
• 页码：106-119
• 文件大小：2031 K

摘要

To identify new genes affecting Tomato bushy stunt virus (TBSV) replication in yeast model host, we are studying protein families, whose members have been identified during previous high throughput screening. In this paper, we have characterized the WW domain-containing protein family from yeast and plants. We find that, in addition to Rsp5 E3 ubiquitin ligase, yeast Wwm1 and Prp40 and three Arabidopsis WW domain-containing proteins are strong inhibitors of TBSV replication. The tombusvirus replicase complex isolated from yeast with down-regulated Wwm1 protein level was more active. Accumulation of viral p92^pol was reduced when Wwm1 was over-expressed, suggesting that the stability of p92^pol might be reduced, as observed with Rsp5. Moreover, replication of two insect RNA viruses is also inhibited by Wwm1 and Rsp5, suggesting that WW domain-containing proteins might have broad regulatory effects on RNA viruses. Thus, artificial antiviral proteins with WW domains could be useful antiviral strategy.