Synthesis and biological activity of carbamate-linked cationic lipids for gene delivery in vitro

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• 作者：Defu Zhi^a ; ^b ; Shubiao Zhang^b ; ^{zsb@dlnu.edu.cn} ; Farooq Qureshi^c ; Yinan Zhao^b ; Shaohui Cui^b ; Bing Wang^b ; Huiying Chen^b ; Yinhuan Wang^d ; Defeng Zhao^a ; ^{zhaodfg@chem.dlut.edu.cn}
• 关键词：Carbamate-linked cationic lipids ; DNA condensation ; Gene delivery ; Transfection efficiency ; Cytotoxicity
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2012
• 期刊代码：10_0960894x
• 类别：ch
• 出版时间：1 June, 2012
• 卷：22
• 期：11
• 页码：3837-3841
• 文件大小：904 K

摘要

We have introduced a convenient synthesis method for carbamate-linked cationic lipids. Two cationic lipids N-[1-(2,3-didodecylcarbamoyloxy)propyl]-N,N,N-trimethylammonium iodide (DDCTMA) and N-[1-(2,3-didodecyl carbamoyloxy)propyl]-N-ethyl-N,N-dimethylammonium iodide (DDCEDMA), with identical length of hydrocarbon chains, alternative quaternary ammonium heads, carbamate linkages between hydrocarbon chains and quaternary ammonium heads, were synthesized for liposome-mediated gene delivery. Liposomes composed of DDCEDMA and DOPE in 1:1 ratio exhibited a lower zeta potential as compared to those made of pure DDCEDMA alone, which influences their DNA-binding ability. pGFP-N2 plasmid was transferred by cationic liposomes formed from the above cationic lipids into Hela and Hep-2 cells, and the transfection efficiency of some of cationic liposomes was superior or parallel to that of two commercial transfection agents, Lipofectamine2000 and DOTAP. Combined with the results of the agarose gel electrophoresis and transfection experiment, the DNA-binding ability of cationic lipids was too strong to release DNA from complex in the transfection, which could lead to relative low transfection efficiency and high cytotoxicity.