Regulation of reproduction- and biomarker-related gene expression by sex steroids in the livers and ovaries of adult female western mosquitofish (Gambusia affinis)
- 作者：Guo-Yong Huang huang_ghy@sina.com ; Guang-Guo Ying ; guangguo.ying@gmail.com ; guang-guo.ying@gig.ac.cn ; Shan Liu ; Yi-Xiang Fang
- 关键词：Cytochrome P4501A ; Endocrine disruption ; Hormone receptor ; Metallothionein ; Vitellogenin
- 刊名：Comparative Biochemistry and Physiology - Part A ; Molecular & Integrative Physiology
- 出版年：2012
- 期刊代码：23_10956433
- 类别：abs
- 出版时间：May, 2012
- 卷：162
- 期：1
- 页码：36-43
- 文件大小：566 K
摘要
To assess the adverse toxicological effects of steroid hormones on western mosquitofish (Gambusia affinis), 180 adult females were exposed to individual or binary combinations of progesterone (1 渭g/L), testosterone (1 渭g/L) and 17尾-estradiol (1 渭g/L) for eight days. The expression patterns of vitellogenin, estrogen receptor, androgen receptor, metallothionein, and cytochrome P450 1A genes in mosquitofish varied according to tissue as well as the specificity of steroids. Treatment by progesterone or testosterone alone inhibited target gene expression in the livers. The expression levels of both vitellogenin A and vitellogenin B mRNAs were up-regulated by17尾-estradiol, and a parallel induction of estrogen receptor 伪 mRNA expression was also observed in the livers. In addition, 17尾-estradiol treatment alone suppressed androgen receptor 伪, metallothionein and cytochrome P450 1A mRNA expression in the livers. In general, multiple hormone treatments had different effects on target gene expression compared with corresponding hormone alone. The results demonstrate that steroid hormones cause multiple biological responses including the expression of vitellogenin, estrogen receptor and androgen receptor mRNA in the hormone signaling pathways and the expression of metallothionein and cytochrome P450 1A mRNA in the xenobiotic signaling pathway.