DNA biosensor based on chitosan film doped with carbon nanotubes
- 作者：Jia Li ; Qian Liu ; Yingju Liu ; Shanchao Liu and Shouzhuo Yao
- 刊名：Analytical Biochemistry
- 出版年：2005
- 期刊代码：93_00032697
- 类别：imm
- 出版时间：2005
- 卷：346
- 期：1
- 页码：107-114
- 文件大小：231 K
摘要
A biosensor based on chitosan doped with carbon nanotube (CNT) was fabricated to detect salmon sperm DNA. Methylene blue (MB) was employed as a DNA indicator. It was found that CNTs can enhance the electroactive surface area threefold (0.28 ± 0.03 and 0.093 ± 0.06 cm2 for chitosan–CNT- and chitosan-modified electrodes, respectively) and can accelerate the rate of electron transfer between the redox-active MB and the electrode. A low detection limit of 0.252 nM fish sperm DNA was achieved, and no interference was found in the presence of 5 μg/ml human serum albumin. The differential pulse voltammetry signal of MB was linear over the fish sperm DNA concentration range of 0.5–20 nM.