Determination of dissociation constants of pharmacologically active xanthones by capillary zone electrophoresis with diode array detection

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• 作者：Wu ; Xiaomu ; Gong ; Suxuan ; Bo ; Tao ; Liao ; Yiping ; Liu ; Huwei
• 关键词：Dissociation constant ; Xanthone ; Capillary zone electrophoresis ; Diode array detection ; Herbal medicine
• 刊名：Journal of Chromatography A
• 出版年：2004
• 期刊代码：47_00219673
• 类别：ch
• 出版时间：December 24, 2004
• 卷：1061
• 期：2
• 页码：217-223
• 文件大小：185 K

摘要

In this article, the dissociation constants (pK_a) of 10 pharmacologically active xanthones isolated from herbal medicine Securidaca inappendiculata were determined by capillary zone electrophoresis with diode array detection. The pK_a values determined by the method based on the electrophoretic mobilities (calculated from migration times) have been proved by the method based on UV absorbance calculated from the online spectra corresponding peaks. No conspicuous difference was observed between the two methods with acceptable reproducibility. Two pK_a values (pK_a1 and pK_a2) were found for four xanthones while generally the 10 compounds possess the pK_a values ranging from 6.4 to 9.2.