Virus-like particles of hepatitis B virus core protein containing five mimotopes of infectious bursal disease virus (IBDV) protect chickens against IBDV

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• 作者：Yong-shan Wang^a ; ^{wangyongshan2001@yahoo.com.cn} ; Wei Ouyang^a ; Xiao-juan Liu^a ; ^b ; Kong-wang He^a ; Sheng-qing Yu^c ; Hai-bin Zhang^b ; Hong-jie Fan^b ; Cheng-ping Lu^b
• 关键词：IBDV ; infectious bursal disease virus ; 5EPIS ; a polypeptide consisting of 5 epitopes ; actually mimotopes ; of IBDV VP2 protein linked in tandem with short amino acid spacers ; HBc ; hepatitis B core protein ; VLP ; virus-like particles
• 刊名：Vaccine
• 出版年：2012
• 期刊代码：89_0264410x
• 类别：imm
• 出版时间：9 March, 2012
• 卷：30
• 期：12
• 页码：2125-2130
• 文件大小：551 K

摘要

Current infectious bursal disease virus (IBDV) vaccines suffer from maternal antibody interference and mimotope vaccines might be an alternative. Previously we demonstrated an IBDV VP2 five-mimotope polypeptide, 5EPIS, elicited protective immunity in chickens. In the current study, the 5epis gene was inserted into a plasmid carrying human hepatitis B virus core protein (HBc) gene at its major immunodominant region site. The recombinant gene was efficiently expressed in Escherichia coli to produce chimeric protein HBc-5EPIS which self-assembles to virus-like particles (VLP). Two-week old specific-pathogen-free chickens were immunized intramuscularly with HBc-5EPIS VLP or 5EPIS polypeptide without adjuvant (50 渭g/injection) on day 0, 7, 14 and 21. Anti-5EPIS antibody was first detected on day 7 and day 21 in HBc-5EPIS and 5EPIS groups, respectively; on day 28, anti-5EPIS titers reached 12,800 or 1600 by ELISA, and 3200 or 800 by virus neutralization assay in HBc-5EPIS and 5EPIS groups, respectively. No anti-5EPIS antibody was detected in the buffer control group throughout the experiment. Challenge on day 28 with a virulent IBDV strain (GX8/99) resulted in 100%, 40.0%and 26.7%survival for chickens immunized with HBc-5EPIS, 5EPIS and buffer, respectively. These data suggest epitope presentation on chimeric VLP is a promising approach for improving mimotope vaccines for IBDV.