Dogs cloned from fetal fibroblasts by nuclear transfer
- 作者：So Gun Hong ; Goo Jang ; Min Kyu Kim ; Hyun Ju Oh ; Jung Eun Park ; Jung Taek Kang ; Ok Jae Koo ; Dae Yong Kim ; Byeong Chun Lee
- 关键词：Fetal fibroblasts ; Dog cloning ; Somatic cell nuclear transfer
- 刊名：Animal Reproduction Science
- 出版年：2009
- 期刊代码：8_03784320
- 类别：abs
- 出版时间：October 2009
- 卷：115
- 期：1-4
- 页码：334-339
- 文件大小：418 K
摘要
Fetal fibroblasts have been considered as the prime candidate donor cells for the canine reproductive cloning by somatic cell nuclear transfer (SCNT) in regard to the future production of transgenic dogs, mainly due to their higher developmental competence and handling advantage in gene targeting. In this study, the cloning efficiency with canine fetal fibroblasts as donor cells was determined. A total of 50 presumptive cloned embryos were reconstructed, activated and transferred into the oviducts of naturally synchronous recipient bitches. While the fusion rate (76.9%) was similar to those of our earlier studies with adult fibroblasts as donor cells (73.9–77.1%), a high cloning efficiency (4.0%; 2 births/50 embryos transferred) was found compared to the previous success rate with adult fibroblasts (0.2–1.8%). The cloned beagles were healthy and genotypically identical to the donor fibroblast cells. This study shows that a fetal fibroblast cell would be an excellent donor for future production of transgenic dogs via gene targeting in this cell followed cloning using SCNT technology.