Inhibitory effect of sesaminol glucosides on lipopolysaccharide-induced NF-κB activation and target gene expression in cultured rat astrocytes
- 作者：Sun Young Lee ; Dong Ju Son ; Yong Kyoung Lee ; Jae Woong Lee ; Hwa Jung Lee ; Young Won Yun ; Tae Youl Ha and Jin Tae Hong
- 关键词：Sesaminol glucosides ; Lipopolysaccharide ; NF-κ ; B ; Inflammatory reaction ; Astrocytes ; Alzheimer's disease
- 刊名：Neuroscience Research
- 出版年：2006
- 期刊代码：53_01680102
- 类别：neu
- 出版时间：October 2006
- 卷：56
- 期：2
- 页码：204-212
- 文件大小：430 K
摘要
The inflammatory reaction plays an important role in the pathogenesis of the neurodegenerative disorder including Alzheimer's disease (AD). Sesame lignan compounds such as sesaminol glucosides (SG) exhibit a range of pharmacological activities including anti-oxidative and anti-inflammatory action. In this study, we tried to elucidate possible effects of SG on lipopolysaccharide (LPS)-induced inflammatory reaction and its underlying mechanism in cultured astrocytes. SG (10–100 μg/ml) inhibited LPS-induced generation of nitric oxide (NO) and reactive oxygen species (ROS), as well as inhibited LPS-induced cytosolic phospholipase A2 (cPLA2), cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) expression dose-dependently. This inhibitory effect of SG on NO and ROS generation was enforced by addition of glutathione (GSH) in culture. In addition, SG prevented LPS-induced DNA binding and transcriptional activity of nuclear factor KappaB (NF)-κB. Consistent with the inhibitory effect on NF-κB activity, SG inhibits phosphorylation and degradation of inhibitory KappaB (IκB), thereby translocation of p50 of NF-κB. These data show that SG has an anti-inflammatory effect through inhibition of NF-κB, and may be a useful agent for prevention of inflammatory disease like AD.