Inhibitory effects of Tabebuia impetiginosa inner bark extract on platelet aggregation and vascular smooth muscle cell proliferation through suppressions of arachidonic acid liberation and ERK1/2 MAPK activation
- 作者：Dong-Ju Son ; Yong Lim ; Young-Hyun Park ; Sung-Keun Chang ; Yeo-Pyo Yun ; Jin-Tae Hong ; Gary R. Takeoka ; Kwang-Geun Lee ; Sung-Eun Lee ; Mi-Ran Kim et al.
- 关键词：Taheebo ; Platelet aggregation ; Arachidonic acid ; Vascular smooth muscle cell ; ERK1/2 ; Atherosclerosis
- 刊名：Journal of Ethnopharmacology
- 出版年：2006
- 期刊代码：26_03788741
- 类别：pha
- 出版时间：3 November 2006
- 卷：108
- 期：1
- 页码：148-151
- 文件大小：331 K
摘要
The antiplatelet and antiproliferative activities of extract of Tabebuia impetiginosa inner bark (taheebo) were investigated using washed rabbit platelets and cultured rat aortic vascular smooth muscle cells (VSMCs) in vitro. n-Hexane, chloroform and ethyl acetate fractions showed marked and selective inhibition of platelet aggregation induced by collagen and arachidonic acid (AA) in a dose-dependent manner. These fractions, especially the chloroform fraction, also significantly suppressed AA liberation induced by collagen in [3H]AA-labeled rabbit platelets. The fractions, especially the chloroform fraction, potently inhibited cell proliferation and DNA synthesis induced by platelet derived growth factor (PDGF)-BB, and inhibited the levels of phosphorylated extracellular signal regulated kinase (ERK1/2) mitogen activated protein kinase (MAPK) stimulated by PDGF-BB, in the same concentration range that inhibits VSMC proliferation and DNA synthesis.