Effects of lactose-β-sitosterol and β-sitosterol on ovalbumin-induced lung inflammation in actively sensitized mice
- 作者：Ji Eun Yuk ; Jin Suk Woo ; Chi-Young Yun ; Ji-Sook Lee ; Joo-Hwan Kim ; Gyu-Yong Song ; Eun Ju Yang ; In Kang Hur ; In Sik Kim
- 关键词：Lactose-β ; -sitosterol ; β ; -sitosterol ; Asthma
- 刊名：International Immunopharmacology
- 出版年：2007
- 期刊代码：123_15675769
- 类别：med
- 出版时间：5 December 2007
- 卷：7
- 期：12
- 页码：1517-1527
- 文件大小：1145 K
摘要
Asthma is a disease marked by chronic lung inflammation and the number of patients suffering from asthma increases annually. Both β-sitosterol (BS) and β-sitosterol glucoside exist in a variety of plants and have anti-tumor, anti-microbial, and immunomodulatory activities. However, the precise role of BS and β-sitosterol glucoside in asthma has not been well understood. The aim of this study was to investigate the inhibitory effects of BS and lactose-BS (L-BS) on the pathophysiological process in ovalbumin-induced asthmatic mice. The total cells and eosinophils in the bronchoalveolar lavage (BAL) fluid markedly decreased (p < 0.05) after L-BS or BS administration (1 mg/kg; i.p.), and the ROS production also decreased in comparison to the asthma control. Histopathological features were detected by performing histochemisty, including H&E and alcian blue & P.A.S staining. Both L-BS and BS mitigated the inflammation by eosinophil infiltration and mucus hypersecretion by goblet hyperplasia. These effects of L-BS were superior to those of BS. L-BS and BS inhibited the increased mRNA and protein expression of IL-4 and IL-5 in the lung tissue and BAL fluid, respectively. The IgE concentration in the BAL fluid and serum was measured by performing ELISA and the ovalbumin-specific IgE in the BAL fluid was uniquely inhibited by L-BS (p < 0.05). The splenocytes were isolated from the normal and asthmatic mice and incubated in the absence and presence of 100 μg/ml ovalbumin, respectively. L-BS blocked the survival rate of the splenocytes of the mice (p < 0.01). This finding indicates the possibility of L-BS and BS as potential therapeutic molecules in asthma and may contribute to the need to improve current therapeutic drugs.