Alteration of intracellular secretory acute phase response proteins expressed in human hepatocyte induced by exposure with interleukin-6

详细信息	查看全文 | 推荐本文 |

• 作者：Katsunori Nakata^a ; ^b ; Ryoichi Saitoh^b ; Jun Amano^b ; Akiyo Koshiyama^a ; Tomoko Ichibangase^a ; Naoaki Murao^b ; Kunihiro Ohta^b ; Yoshinori Aso^b ; Masaki Ishigai^b ; Kazuhiro Imai^a ; ^{k-imai@musashino-u.ac.jp}
• 关键词：IL-6 ; Interleukin-6 ; FD ; fluorogenic derivatization ; LC ; high-performance liquid chromatography ; MS/MS ; tandem mass spectrometry ; DAABD-Cl ; 7-chloro-N-[2-(dimethylamino)ethyl]-2 ; 1 ; 3-benzoxadiazole-4-sulfonamide
• 刊名：Cytokine
• 出版年：2012
• 期刊代码：97_10434666
• 类别：imm
• 出版时间：August, 2012
• 卷：59
• 期：2
• 页码：317-323
• 文件大小：599 K

摘要

Interleukin-6 (IL-6) is a principal proinflammatory cytokine inducing the acute phase response in various tissues, including liver. Here, we adopt the FD-LC-MS/MS method, consisting of fluorogenic derivatization (FD), separation by liquid chromatography (LC), and identification of proteins by LC-tandem mass spectrometry (MS/MS), to reveal how exposure to IL-6 alters temporally the intracellular secretory acute phase response (sAPR) proteins expressed in human hepatocytes as compared to non-exposure. Nine altered sAPR proteins were identified in cultures in response to IL-6. Seven of them (serum amyloid A protein, haptoglobin, fibrinogen 伪 chain, fibrinogen 尾 chain, fibrinogen 纬 chain, 伪₁-acid glycoprotein and 伪₁-antitrypsin) were significantly increased and two (尾₂-glycoprotein 1 and transferrin) were significantly decreased in response to IL-6. In addition, the transmission speed of transferrin might be much faster than the other sAPR proteins. These results suggest a different molecular mechanism for protein synthesis and the secretory pathway among the sAPR proteins. In this study, we observed the simultaneously and temporally altered expression of sAPR proteins which had been induced by exposure to IL-6 in human hepatocytes, in contrast to previous reports, in all of which the proteins were tested from the time they were secreted into the medium from the cells.