The CD14 region spanning amino acids 57脙脙脙脙脙脙64 is critical for interaction with the extracellular Toll-like receptor 2 domain

详细信息	查看全文 | 推荐本文 |

• 作者：Iwaki ; Daisuke ; Nishitani ; Chiaki ; Mitsuzawa ; Hiroaki ; Hyakushima ; Naoki ; Sano ; Hitomi ; Kuroki ; Yoshio
• 关键词：CD14 ; Toll-like receptor 2 ; Peptidoglycan
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2005
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：March 4, 2005
• 卷：328
• 期：1
• 页码：173-176
• 文件大小：241 K

摘要

CD14 has been shown to enhance Toll-like receptor 2 (TLR2)-mediated signaling in response to peptidoglycan. Anti-CD14 monoclonal antibody MEM-18, whose epitope was located at the amino acid residues 57芒芒芒64, blocked the binding of sCD14 to the recombinant soluble form of the extracellular TLR2 domain (sTLR2). The deletion mutant sCD14^{芒芒57芒芒芒64} lacking the amino acid residues 57芒芒芒64 failed to bind to sTLR2. Cotransfection of wild type mCD14 but not mCD14^{芒芒57芒芒芒64} with TLR2 enhanced NF-芒芒B activation in response to peptidoglycan. These results indicate that the CD14 region spanning amino acids 57芒芒芒64 is critical for interacting with TLR2 and enhancing TLR2-mediated peptidoglycan signaling.