Thiol specific oxidative stress response in Mycobacteria
- 作者：Nirpjit S. Dosanjh ; Mamta Rawat ; Ji-Hae Chung and Yossef Av-Gay
- 关键词：Mycothiol ; Diamide ; Mycobacteria ; Disulfide
- 刊名：FEMS Microbiology Letters
- 出版年：2005
- 期刊代码：34_03781097
- 类别：imm
- 出版时间：2005
- 卷：249
- 期：1
- 页码：87-94
- 文件大小：248 K
摘要
The cellular response of mycobacteria to thiol specific oxidative stress was studied in Mycobacterium bovis BCG cultures. Two-dimensional gel electrophoresis revealed that upon diamide treatment at least 60 proteins were upregulated. Fourteen of these proteins were identified by MALDI-MS; four proteins, AhpC, Tpx, GroEL2, and GroEL1 are functionally related to oxidative stress response; eight proteins, LeuC, LeuD, Rv0224c, Rv3029c, AsnB, Rv2971, PheA and HisH are classified as part of the bacterial intermediary metabolism and respiration pathways; protein EchA14 belong to lipid metabolism, and NrdE, belongs to the mycobacterial information pathway category. Reverse transcription followed by quantitative real time PCR in response to diamide stress demonstrated that protein expression is directly proportional to the corresponding gene transcription.