Neuroprotective effects of phlorotannins isolated from a brown alga, Ecklonia cava, against H₂O₂-induced oxidative stress in murine hippocampal HT22 cells

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• 作者：Sung-Myung Kang^a ; ¹ ; Seon-Heui Cha^b ; ¹ ; Ju-Young Ko^a ; Min-Cheol Kang^a ; Daekyung Kim^c ; Soo-Jin Heo^d ; Jin-Soo Kim^e ; ^f ; Min Soo Heu^e ; ^f ; Yong-Tae Kim^g ; Won-Kyo Jung^h ; You-Jin Jeon^a ; ^{youjinj@jejunu.ac.kr}
• 关键词：Neuroprotection ; Ecklonia cava ; Phlorotannin ; Oxidative stress
• 刊名：Environmental Toxicology and Pharmacology
• 出版年：2012
• 期刊代码：13_13826689
• 类别：pha
• 出版时间：July, 2012
• 卷：34
• 期：1
• 页码：96-105
• 文件大小：1320 K

摘要

Exposure of neurons to hydrogen peroxide (H₂O₂) results in oxidative stress and the activation of a cascade of intracellular toxic events resulting in oxidation, lipid peroxidation, and Ca²⁺ elevation, ultimately resulting in cell death. In this study, we attempted to characterize the neuroprotective effects of phlorotannins isolated from Ecklonia cava, including phloroglucinol, eckol, triphloroethol A, eckstolonol, and dieckol, against H₂O₂-induced cell damage in murine hippocampus neuronal (HT22) cells. We measured the reactive oxygen species (ROS) and lipid peroxidation levels and evaluated the resultant cell death and alterations in Ca²⁺-concentrations. All phlorotannins were to scavenge intracellular ROS and repress ROS accumulation, thus preventing lipid peroxidation. Consquently, all phlorotannins reduced H₂O₂-induced cell death in HT22 cells. Moreover, phlorotannins inhibited H₂O₂-induced Ca²⁺ release. This study provides a new useful strategy for preventing neuronal H₂O₂-induced oxidative stress.