Anti-inflammatory activity of Chrysanthemum zawadskii var. latilobum leaf extract through haem oxygenase-1 induction

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• 作者：Younghwa Kim^a ; Jiyoung Han^b ; Jeehye Sung^a ; Misun Sung^a ; Youngmin Choi^a ; Heon-Sang Jeong^a ; Junsoo Lee^a ; ^{junsoo@chungbuk.ac.kr}
• 关键词：Chrysanthemum zawadskii ; Anti-inflammation ; Haem oxygenase-1 ; Nitric oxide
• 刊名：Journal of Functional Foods
• 出版年：2012
• 期刊代码：P18_17564646
• 类别：bio
• 出版时间：April, 2012
• 卷：4
• 期：2
• 页码：474-479
• 文件大小：316 K

摘要

Chrysanthemum zawadskii var. latilobum (CZ) has been widely used as a tea in Korea. In this study, we investigated the involvement of anti-inflammatory haem oxygenase-1 (HO-1) in the inhibitory activity of a CZ leaf extracts on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. At the highest concentration of CZ leaf extract (50 渭g/mL), NO production was 10%of that in LPS-stimulated macrophages, whereas iNOS protein expression was 5%of that in LPS-stimulated macrophages. CZ leaf extract also inhibited iNOS mRNA expression in a concentration-dependant manner, and at 50 渭g/mL, iNOS mRNA expression was approximately 15%of that in LPS-stimulated cells. The CZ leaf extract induced HO-1 expression in a dose-dependent manner, and blocking HO-1 activity abolished the anti-inflammatory effect of CZ leaf extract. These results suggest that CZ leaf extract has a potent anti-inflammatory activity in RAW264.7 cells through the induction of HO-1.