Isolated PSCs from Sprague Dawley rats were co-cultured with different doses of octreotide (1.25, 2.5, 5, 10, 20, and 40 ng/mL). PSC proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide at 48, 72, and 96 h. The聽伪-smooth muscle actin (伪-SMA) and collagen I expressions of PSCs were detected by immunohistochemistry and reverse-transcriptase polymerase chain reaction. Rat heterotopic pancreaticoduodenal transplantation was performed with and without octreotide treatment (0.01 mg/kg). Pancreas grafts were harvested at postoperative d 1, 3, 5, and 7. Hematoxylin-eosin staining, Masson鈥檚 trichrome staining, and immunohistochemical staining for 伪-SMA, collagen I, and tumor growth factor-尾1 (TGF-尾1) were performed.
Octreotide at a concentration of >20 ng/mL significantly inhibited PSC activation and proliferation in聽vitro. Inflammatory infiltration was reduced in the octreotide group in聽vivo, and the expression levels of 伪-SMA, collagen I, and TGF-尾1 were also lower, with statistic significant difference or not. Masson鈥檚 trichrome staining showed a decrease in collagen deposition with octreotide treatment.
Octreotide effectively inhibits PSC activation and proliferation in聽vitro, but has a limited inhibitory effect on the development of pancreas graft fibrosis.