Human cytomegalovirus preferentially infects the neoplastic epithelium of colorectal cancer: A quantitative and histological analysis

详细信息	查看全文 | 推荐本文 |

• 作者：Hsin-Pai Chen^a ; ^b ; ^c ; ⁿ ; Jeng-Kai Jiang^b ; ^d ; ⁿ ; Cheng-Yu Chen^a ; ^c ; Teh-Ying Chou^c ; ^e ; Yen-Chung Chen^f ; Ya-Ting Chang^g ; Shiou-Fu Lin^e ; Chia-Hao Chan^g ; Chih-Yung Yang^h ; ⁱ ; Chi-Hung Lin^h ; ⁱ ; ^j ; Jen-Kou Lin^b ; ^d ; Wen-Long Cho^c ; ^k ; ^{wenlong@ym.edu.tw} ; Yu-Jiun Chan^g ; ^l ; ^m ; ^{yjchan@vghtpe.gov.tw}
• 关键词：HCMV ; human cytomegalovirus ; CRC ; colorectal cancer ; ISH ; in situ hybridization ; VGHTPE ; Taipei Veterans General Hospital
• 刊名：Journal of Clinical Virology
• 出版年：2012
• 期刊代码：150_13866532
• 类别：med
• 出版时间：July, 2012
• 卷：54
• 期：3
• 页码：240-244
• 文件大小：572 K

摘要

Background

It has long been suggested that human cytomegalovirus (HCMV) might be involved in human oncogenesis. However, whether HCMV was associated with colorectal cancer (CRC) was still controversial.

Objective

To clarify whether HCMV specifically infects the tumorous tissue of CRC.

Study design

Paired tumor and adjacent non-neoplastic CRC specimens were collected from 163 patients. HCMV DNA was detected and quantified through PCR and quantitative real-time PCR. Virus location was determined by in situ hybridization (ISH) of formalin-fixed paraffin-embedded tissue sections with an HCMV-specific probe.

Results

By PCR, HCMV DNA was detected in 42.3%(69/163) of the tumor specimens, while only 5.6%(14/163) samples of adjacent non-neoplastic tissue were positive for HCMV (p < 0.0001). Quantitative real-time PCR in 54 sample pairs revealed significantly higher viral copies in the tumor specimens than the adjacent non-neoplastic tissue specimens (p < 0.001). By ISH, the nucleic acids of HCMV were detected in the cytoplasm of neoplastic epithelium. No hybridization was detected in the inflammatory infiltrates, submucosa, or other stromal tissues.

Conclusions

HCMV preferentially infects the tumor epithelium of CRC. How the virus subsists in and interacts with the microenvironment of tumor epithelium of CRC should be studied.