Effect of microwave irradiation pretreatment of cow dung compost on bio-hydrogen process from corn stalk by dark fermentation
- 作者：Zhao-Xia Song ; Zhi-Yuan Wang ; Li-Yang Wu ; Yao-Ting Fan ; yt.fan@zzu.edu.cn ; ytfan1207@yahoo.com.cn ; Hong-Wei Hou
- 关键词：Bio-hydrogen production ; Cow dung compost ; Microwave irradiation ; PCR-DGGE ; Microbial characteristics
- 刊名：International Journal of Hydrogen Energy
- 出版年：2012
- 期刊代码：42_03603199
- 类别：ch
- 出版时间：April, 2012
- 卷：37
- 期：8
- 页码：6554-6561
- 文件大小：473 K
摘要
The bio-hydrogen production potential from corn stalk was significantly affected by microwave irradiation pretreatment of cow dung compost in batch tests. The maximum hydrogen yield of 144.3聽ml/g-corn stalk and hydrogen production rate of 3.6聽ml/g-corn stalk聽h鈭? were observed using the pretreated compost by microwave radiation of 1.5聽min at fixed Na2CO3 dosage of 800聽mg/l, Fe dosage of 400聽mg/l, substrate concentration of 20聽g/l, which increased about 99.6%and 85.2%compared with that of the control. The effects of microwave irradiation on microbial characteristics were further discussed by Atomic Force Microscope (AFM), determination of protein content and PCR-DGGE. The four dominant hydrogen-producing strains had been isolated and confirmed to be Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus subtilis and Enterococcus faecium, respectively. The diversity and symbiosis relations of the mixed bacteria were also observed in fermentation hydrogen production process.