Constructing a novel Nanodevice powered by δ-free F_oF₁-ATPase

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• 作者：Ting Su ; Yuanbo Cui ; Xiaoai Zhang ; Xiaolong Liu ; Jiachang Yue ; Ning Liu and Peidong Jiang
• 关键词：Nanodevice ; ATPase motor ; Assembly ; Actin filament ; Chromatophores ; PMF
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2006
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：1 December 2006
• 卷：350
• 期：4
• 页码：1013-1018
• 文件大小：733 K

摘要

A Nanodevice was constructed by δ-free F_oF₁-ATPase within chromatophores and actin filaments through biotinlipid–streptavidin–biotin-(AC₅)₂Sulfo-OSu system. One actin filament linking with many chromatophores functions as the Nanodevice body and many δ-free F_oF₁-ATPase as the Nanodevice motors. Movement of the Nanodevice was observed directly by fluorescence microscopy with CCD camera after illumination. The moving speed was about 2.17–24.43 μm/s for various length Nanodevices and most of them were stopped by adding CCCP. This means that the Nanodevice was driven by PMF (proton-motive force) in the cooperating δ-free F_oF₁-ATPase. From bioengineering point of view, the cooperation of F_oF₁-ATPase is a very important research field in the future.