Preparation, characterization and anticoagulant activity in vitro of heparin-like 6-carboxylchitin derivative

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• 作者：Jianhong Yang^a ; ^b ; ^c ; ^{yangshenyang3@163.com} ; Jun Cai^b ; Kang Wu^c ; Dinglong Li^a ; Ying Hu^b ; Guoxiang Li^c ; Yumin Du^c
• 关键词：Chitin ; 6-Carboxychitin ; Heparin-like ; Sulfation ; Anticoagulant activity
• 刊名：International Journal of Biological Macromolecules
• 出版年：2012
• 期刊代码：62_01418130
• 类别：abs
• 出版时间：1 May, 2012
• 卷：50
• 期：4
• 页码：1158-1164
• 文件大小：672 K

摘要

A series of heparin-like 6-carboxylchitin derivatives with different N-acetyl group and sulfate group contents were prepared. Their structures were characterized by element analysis, FT-IR, ¹³C NMR, and gel permeation chromatography. Their anticoagulant activity in vitro was investigated for human plasma with respect to activated partial thromboplastin time (APTT). The results showed all 6-carboxylchitin derivatives prolonged APTT within the scope of studied degree of sulfation (0.28-1.03) and Mws (4.3-13.7 kDa). Their anticoagulant activity strongly depended on their structures. 3,6-O-sulfated group promoted the anticoagulant activity. Only incorporation of N-sulfated group into deacetylated 6-carboxylchitin could not improve the anticoagulant activity. But, N-sulfated group and O-sulfated group had the synergistic action, and N-sulfated group could promote the anticoagulant activity for the N,O-sulfated chitin derivatives. In addition, acetyl group took a role in the anticoagulant activity, too.