Lipase-coated K₂SO₄ micro-crystals: Preparation, characterization, and application in biodiesel production using various oil feedstocks

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• 作者：Jingjing Zheng ; Li Xu ; Yun Liu ; ^{liuyunprivate@sina.com} ; Xiaoyan Zhang ; Yunjun Yan ; ^{yanyunjun@mail.hust.edu.cn}
• 关键词：Protein-coated micro-crystals (PCMCs) ; Biodiesel ; Response surface methodology (RSM) ; Property characterization ; Pseudomonas cepacia lipase (PS)
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：April, 2012
• 卷：110
• 期：Complete
• 页码：224-231
• 文件大小：532 K

摘要

This study investigated the preparation and characteristics of protein-coated microcrystals (PCMCs) from Pseudomonas cepacia lipase (PS) and K₂SO₄, and their application in biodiesel synthesis, via single factorial experiments and response surface methodology (RSM), the optimized PCMC-PS exhibited high activity and stability; the optimal temperature was 60 掳C (which gave 99.83%conversion), although fairly high activity was exhibited after incubation at different temperatures (25-70 掳C). The organic solvents stability of the PCMC-PS was improved, and it significantly reduced ethanol toxicity. Circular dichroism (CD) analysis revealed the correlation between the conformation and the enzyme activity. The morphology of the PCMC-PS was also confirmed via scanning electron microscopy (SEM). When catalyzed by PCMC-PS, above 83%biodiesel yield was obtained for most of the seven oils tested. The PCMC-PS (washed with hexane) activity remained relatively stable after eight batch reactions, with only a 15.73%reduction in the conversion (from 99.02%to 83.29%).