Chromatographic evaluation of perphenylcarbamoylated β-cyclodextrin bonded stationary phase for micro-high performance liquid chromatography and pressurized capillary electrochromatography

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• 作者：Ailing Zhou ; Xianyu Lv ; Yunxuan Xie ; Chao Yan and Ruyu Gao
• 关键词：Chiral stationary phase (CSP) ; Perphenylcarbamoylated β ; -cyclodextrin ; α ; -Aminophosphonate ; Micro-high performance liquid chromatography ; Pressurized capillary electrochromatography
• 刊名：Analytica Chimica Acta
• 出版年：2005
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：2005
• 卷：547
• 期：2
• 页码：158-164
• 文件大小：283 K

摘要

Application of mono (6^A-N-ethylenediamine-6^A-deoxy) perphenylcarbamoylated β-cyclodextrin (β-CD) bonded stationary phase (CSP) in micro-high performance liquid chromatography (micro-HPLC) and pressurized capillary electrochromatography (p-CEC) was firstly presented. A series of racemic α-amidophosphonates were resolved in reversed- and normal-phase modes on this CSP. The investigated chromatographic parameters include retention factor (k′), separation factor (α) and resolution (Rs) of solutes. In addition, the structural variation of the solutes and the experimental factors affecting chiral separations have been examined, including the percentage of alcohol modifier, the linear velocity (u) of the mobile phase, electrical field strength, etc. Baseline separation was achieved for most of the entities. Hydrophobic interaction, steric effect and π–π interaction contribute to the possible mechanism. Comparative results indicate that higher Rs value up to 3.1 was found in micro-HPLC, higher efficiency up to 29,970 in p-CEC.