Rapid amperometric detection of coliforms based on MWNTs/Nafion composite film modified glass carbon electrode
- 作者：Yuxiao Cheng ; Yajun Liu ; Jingjing Huang ; Yuezhong Xian ; Wen Zhang ; Zhonghai Zhang ; Litong Jin
- 关键词：Multi-wall carbon nanotubes ; Nafion ; Coliforms ; Amperometric detection ; β ; -Galactosidase ; p-Aminophenol
- 刊名：Talanta
- 出版年：2008
- 期刊代码：106_00399140
- 类别：ch
- 出版时间：15 March 2008
- 卷：75
- 期：1
- 页码：167-171
- 文件大小：141 K
摘要
A multi-wall carbon nanotubes (MWNTs)/Nafion modified glassy carbon electrode (GCE) was fabricated for the rapid amperometric detection of coliforms, represented by Escherichia coli (E. coli). In the bacterial solution, β-galactosidase which was used as an indicator of coliforms reacted with substrate, p-aminophenol-β-galactopyranoside (PAPG), and produced p-aminophenol (PAP). PAP was detected by MWNTs/Nafion modified GCE. Due to the cation-exchange capacity of Nafion and the electrocatalytic ability of MWNTs, the detection sensitivity of PAP was improved and the detection time of coliforms was shortened. The bacterial can be detected within 5 h ranging from 10 to 104 cfu/mL. The MWNTs/Nafion modified GCE was easy to be constructed and regenerated. To our best knowledge, it was the first time to use MWNTs/Nafion modified GCE to detect the concentration of coliforms.