Development of Cell Perfusion Device and <sup>31sup>P NMR Spectrum Test
- 作者：Zao Zeng<sup>1sup><sup> ; sup><sup>Zengzao2006@126.comsup> ; Jie Liang<sup>1sup> ; Qian-zhu Li<sup>1sup> ; Jian-wei Zhou<sup>2sup><sup> ; sup><sup>jianweizhou_2000@yahoo.comsup>
- 关键词：Cell Perfusion device ; 31P NMR ; Energy metabolism
- 刊名：Energy Procedia
- 出版年：2012
- 期刊代码：P136_18766102
- 类别：pw
- 出版时间：2012
- 卷：17
- 期：Complete
- 页码：1960-1964
- 文件大小：466 K
摘要
To set up a device of cell perfusion, and use the method of cell perfusion -NMR to monitor the <sup>31sup>P spectrum in cells. The result has shown the temperature and pressure and flow rate of the device were invariable relatively and could be controlled. The method of cell perfusion -NMR can monitor the <sup>31sup>P spectrum online, and can Speculate changes of energy metabolism in cells under drug stimulation.