Toxoplasma gondii: Protective immunity against toxoplasmosis with recombinant actin depolymerizing factor protein in BALB/c mice

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• 作者：Xiangsheng Huang¹ ; Jianhua Li ; ¹ ; ^{Jianhuali7207@163.com} ; Guocai Zhang ; Pengtao Gong ; Ju Yang ; Xichen Zhang ; ^{Zhangxic@public.cc.jl.cn}
• 关键词：Toxoplasma gondii ; Recombinant protein ; Actin depolymerizing factor ; BALB/c mice
• 刊名：Experimental Parasitology
• 出版年：2012
• 期刊代码：99_00144894
• 类别：imm
• 出版时间：March, 2012
• 卷：130
• 期：3
• 页码：218-222
• 文件大小：402 K

摘要

Toxoplasmosis is one of the most world-wide spread zoonosis representing a very serious clinical and veterinary problem. There is still need for vaccines for toxoplasmosis. In the present study, we evaluated the protective efficacy of a recombinant actin depolymerizing factor (ADF) subunit vaccine against Toxoplasma gondii infection in BALB/c mice. The recombinant T. gondii ADF protein (rADF) was expressed in Escherichia coli and used as antigens for BALB/c mice immunization. The results indicated that specific antibody and the increased percentage of CD4⁺ T lymphocyte were found in vaccinated BALB/c mice with rADF, when compared with adjuvant or PBS groups. After challenged with T. gondii (RH strain) tachyzoites, the survival time of the mice in rADF group was longer than those in the control group. The numbers of brain cysts of the mice in rADF group reduced significantly when compared with those in control groups (P < 0.05), and the rate of reduction could reach to around 30%. These results suggest that rADF can generate protective immunity against T. gondii infection in BALB/c mice.