A novel hydrogen peroxide sensor based on hemoglobin immobilized PAn-SiO₂/DTAB composite film

详细信息	查看全文 | 推荐本文 |

• 作者：Bin Chen ; Hui Wang ; Haiyan Zhang ; Zhixian He ; Sijing Zhang ; Ting Liu ; Yuanzhen Zhou ; ^{zhouyuanzhen@xauat.edu.cn} ; ^{zyz1289@126.com}
• 关键词：Hydrogen peroxide sensor ; Hemoglobin ; Polyaniline ; Silica ; Dodecyltrimethylammonium bromide
• 刊名：Journal of Molecular Liquids
• 出版年：2012
• 期刊代码：61_01677322
• 类别：ch
• 出版时间：July, 2012
• 卷：171
• 期：Complete
• 页码：23-28
• 文件大小：1031 K

摘要

A novel hydrogen peroxide (H₂O₂) sensor based on hemoglobin (Hb) immobilized polyaniline-silica (PAn-SiO₂) and dodecyltrimethylammonium bromide (DTAB) composite film modified carbon paste electrode (CPE) was fabricated and the direct electron transfer of immobilized Hb was investigated. On Hb/PAn-SiO₂/DTAB/CPE, Hb displayed a fast electron transfer process accompanied with one proton participate in and its electron transfer rate constant which was estimated at 0.94 s^鈭?#xA0;1. Thus, the proposed biosensor exhibited a high sensitivity and excellent electrocatalytic activity to the reduction of H₂O₂. The catalytic reduction current of H₂O₂ was proportional to H₂O₂ concentration in the range of 0.09 to 2.8 mM with the detection limit of 0.03 mM. The apparent Michaelis-Menten constant (K_m^app) of the biosensor was calculated to be 0.275 mM, exhibiting high enzymatic activity and affinity. With the characteristics above, the sensor in this paper can be potentially applied in other reactive oxygen species as well.