Antitumor effects and chemical compositions of Eupolyphaga sinensis Walker ethanol extract

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• 作者：Gang-feng Ge^a ; Chen-huan Yu^b ; Bing Yu^c ; Zhen-hua Shen^c ; Dong-liang Zhang^c ; Qiao-feng Wu^c ; ^{Jellycook2002@163.com}
• 关键词：ESEE ; Eupolyphaga sinensis Walker ethanol extract ; Fu ; fluorouracil ; NC ; normal control ; MC ; model control ; H22 tumor bearing mice ; MC  ; +  ; Fu ; H22 tumor bearing mice treated with fluorouracil ; MC  ; +  ; ESEE ; H22 tumor bearing mice treated wi
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：7 May, 2012
• 卷：141
• 期：1
• 页码：178-182
• 文件大小：933 K

摘要

Ethnopharmacological relevance

Eupolyphaga sinensis Walker popularly known as 鈥減referred drug to regulate blood flow鈥?are traditionally used in folk medicine in the treatment of ecchymoma, posttraumatic wound, hepatic fibrosis and tumor.

Aim of the study

To characterize chemical compositions and to evaluate the antitumor and immunomodulatory of Eupolyphaga sinensis Walker ethanol extract (ESEE) in hepatocarcinoma H₂₂ bearing mice.

Materials and methods

ESEE was obtained by ethanol reflux extraction and analyzed by gas chromatography-mass spectrometry (GC-MS) after methylation. ICR mice were treated with ESEE for 14 consecutive days at doses of 31 mg/kg (low-dose), 62 mg/kg (mid-dose) and 124 mg/kg (high-dose) after H₂₂ tumor cells were implanted. At the end of the experiments, the tumor weight of each mouse was measured. Levels of serum TNF-伪 and IFN-纬 was assayed by ELISA. Protein expressions of Bax, Bcl-2 and caspases-3 were detected by immunohistochemistry.

Results

Chemical analysis revealed the presence of 6 components that account for 97.55%of fatty acids, indicating the occurrence of saturated and polyunsaturated fatty acids. Oral administration of ESEE could inhibit tumor growth, promote Th1 type cytokine productions (TNF-伪 and IFN-纬) and induce apoptosis of hepatocarcinoma via increase of Bax/Bcl-2 ratio and activation of caspases-3. Oral administration of ESEE in a dosage of 6.2 g/kg did not lead to toxic effects in mice.

Conclusions

ESEE was effective in inhibiting tumor growth in vivo and could also serve as immunoadjuvant for tumor therapy.