Methyl-尾-cyclodextrin enhanced biodegradation of polycyclic aromatic hydrocarbons and associated microbial activity in contaminated soil

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• 作者：Mingming Sun^a ; ^{mmsun@issas.ac.cn} ; Yongming Luo^a ; Peter Christie^b ; Zhongjun Jia^a ; Zhengao Li^a ; Ying Teng^a ; ^{yteng@issas.ac.cn}
• 关键词：polycyclic aromatic hydrocarbons ; methyl-尾-cyclodextrin ; biodegradation ; Paracoccus sp. strain HPD-2 ; microbial activity
• 刊名：Journal of Environmental Sciences
• 出版年：2012
• 期刊代码：8_10010742
• 类别：env
• 出版时间：May, 2012
• 卷：24
• 期：5
• 页码：926-933
• 文件大小：185 K

摘要

The contamination of soils by polycyclic aromatic hydrocarbons (PAHs) is a widespread environmental problem and the remediation of PAHs from these areas has been a major concern. The effectiveness of many in situ bioremediation systems may be constrained by low contaminant bioavailability due to limited aqueous solubility or a large magnitude of sorption. The objective of this research was to evaluate the effect of methyl-尾-cyclodextrin (MCD) on bioaugmentation by Paracoccus sp. strain HPD-2 of an aged PAH-contaminated soil. When 10%(W/W) MCD amendment was combined with bioaugmentation by the PAH-degrading bacterium Paracoccus sp. strain HPD-2, the percentage degradation of total PAHs was significantly enhanced up to 34.8%. Higher counts of culturable PAH-degrading bacteria and higher soil dehydrogenase and soil polyphenol oxidase activities were observed in 10%(W/W) MCD-assisted bioaugmentation soil. This MCD-assisted bioaugmentation strategy showed significant increases (p < 0.05) in the average well color development (AWCD) obtained by the BIOLOG Eco plate assay, Shannon-Weaver index (H) and Simpson index (纬) compared with the controls, implying that this strategy at least partially restored the microbiological functioning of the PAH-contaminated soil. The results suggest that MCD-aided bioaugmentation by Paracoccus sp. strain HPD-2 may be a promising practical bioremediation strategy for aged PAH-contaminated soils.