Immune responses of zebrafish (Danio rerio) induced by bath-vaccination with a live attenuated Vibrio anguillarum vaccine candidate
- 作者：Zhihui Zhang ; Haizhen Wu ; wuhzh@ecust.edu.cn ; Jingfan Xiao ; Qiyao Wang ; Qin Liu ; Yuanxing Zhang
- 关键词：Bath-vaccination ; Danio rerio ; Immunoprotection ; Live attenuated vaccine ; Vibrio anguillarum
- 刊名：Fish & Shellfish Immunology
- 出版年：2012
- 期刊代码：115_10504648
- 类别：abs
- 出版时间：July, 2012
- 卷：33
- 期：1
- 页码：36-41
- 文件大小：460 K
摘要
A fish vaccine candidate, live attenuated Vibrio anguillarum, which can protect fish from vibriosis, was established in our laboratory. In this study, the protective immunological mechanism of live attenuated V.聽anguillarum was investigated in zebrafish as a model animal. After bath-vaccinated with the live attenuated strain, zebrafish were challenged with wild pathogenic strain to test the immunoprotection of the live attenuated strain. As the results, specific antibody response of fish against V.聽anguillarum was found to gradually increase during 28 days post-vaccination, and remarkable protection was showed with a high relative protection survival (RPS) of about 90%. Moreover, the vaccination changed the expressions of several immune-related genes in the spleens and livers of zebrafish. Among them, the expressions of pro-inflammatory factors such as IL-1 and IL-8 were tenderly up-regulated with about 3-4 fold in 1-7 days聽post-vaccination, while MHC II rose to a peak level of 4-fold in 7th day post-vaccination. These results gave some important messages about the mechanism of specific protection induced by live attenuated V.聽anguillarum and showed the availability of zebrafish model in the evaluation of the vaccine candidate.