Chromogenic platform based on recombinant Drosophila melanogaster acetylcholinesterase for visible unidirectional assay of organophosphate and carbamate insecticide residues

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• 作者：Zheng Han^a ; Chensen Chi^b ; Bing Bai^a ; Gang Liu^a ; Qinxiong Rao^a ; Shaojie Peng^c ; Hong Liu^d ; Zhihui Zhao^a ; Dabing Zhang^b ; Aibo Wu^a ; ^{wuaibo@saas.sh.cn}
• 关键词：Chromogenic platform ; Visible unidirectional assay ; Insecticides ; On-site analysis
• 刊名：Analytica Chimica Acta
• 出版年：2012
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：30 March, 2012
• 卷：720
• 期：Complete
• 页码：126-133
• 文件大小：906 K

摘要

In this study we propose a chromogenic platform for rapid analysis of organophosphate (OP) and carbamate (CM) insecticide residues, based on recombinant Drosophila melanogaster acetylcholinesterase (R-DmAChE) as enzyme and indoxyl acetate as substrate. The visible chromogenic strip had the advantages identical to those of commonly used lateral flow assays (LFAs) with utmost simplicity in sample loading and result observation. After optimization, depending on the color intensity (CI) values, the well-established assay has the capabilities of both qualitative measurement via naked eyes and quantitative analysis by colorimetric reader with the desirable IC₅₀ values against the tested six insecticides (0.06 渭g mL^鈭? of carbofuran, 0.28 渭g mL^鈭? of methomyl, 0.03 渭g mL^鈭? of dichlorvos, 31.6 渭g mL^鈭? of methamidophos, 2.0 渭g mL^鈭? of monocrotophos, 6.3 渭g mL^鈭? of omethoate). Acceptable matrix effects and satisfactory detection performance were confirmed by in-parallel LC-MS/MS analysis in different vegetable varieties at various spiked levels of 10^鈭? to 10¹ 渭g g^鈭?. Overall, the testified suitability and applicability of this novel platform meet the requirements for practical use in food safety management and environmental monitoring, especially in the developing world.