Sulfhydryl oxidase modified composite electrode for the detection of reduced thiolic compounds
- 作者：Suna Timur ; Dilek Odaci ; Ayse Dincer ; Figen Zihnioglu ; Azmi Telefoncu ; Lo Gorton
- 关键词：Biosensor ; Sulfhydril oxidase (SOX) ; Reduced thiols ; Post-column enzyme based biosensor
- 刊名：Sensors and Actuators B ; Chemical
- 出版年：2007
- 期刊代码：161_09254005
- 类别：et
- 出版时间：16 July 2007
- 卷：125
- 期：1
- 页码：234-239
- 文件大小：313 K
摘要
Thiol (SH) groups play a remarkable role in the cell with their redox state affecting the activity and structure of many enzymes, receptors and transcription factors. In the present work, carbon paste electrodes modified with sulfhydryl oxidase (SOX) were prepared. The proposed biosensor was based on the monitoring of the produced peroxide concentration that correlates with the concentration of reduced thiols during the enzymatic reaction. As well as analytical characteristics, optimization in terms of amount of enzyme, pH and temperature were carried out. After that, thiolic compounds (reduced/oxidized glutathione, cysteine, homocysteine, N-acetylcysteine, dl-dithiotreitol) were separated by HPLC and the performance of the sulfhydril oxidase SOX biosensor as a post-column electrochemical detector was investigated for the analysis of these compounds. All data were given as a comparison of two systems; biosensor and diode array detector (DAD).