The response of biosensor was measured as a function of selected organic solvents (methanol, ethanol and isopropanol) containing phosphate buffer solution (pH = 6.5) at 鈭?#xA0;100 mV against a reference Hg鈭g2Cl2鈭? M KCl electrode. Addition of 10%of methanol and ethanol increased, while higher volume fractions decreased the biosensor response. On the other hand isopropanol (10%) decomposed the photopolymeric membrane. Therefore, the determination of glucose in alcoholic beverages has been performed in phosphate buffer with 10%of ethanol. The linear range was obtained up to 0.8 mM (R2 = 0.9925) for mixed solvents, as well as phosphate buffer (R2 = 0.9996).
The simple, fast and easy preparation of the biosensor, high activity and long life, if glucose was immobilized in mixed polymeric photocurable membrane, and reasonably accurate glucose determination, make the proposed procedure appropriate for quality control of the alcoholic beverages in mixed solvents.