Radial glia in the cerebellum of adult teleost fish: implications for the guidance of migrating new neurons

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• 作者：G.K.H. Zupanc^a ; ^b ; ^c ; ^{g.zupanc@neu.edu} ; R.F. S&icirc ; rbulescu^c ; I. Ilie艧^c
• 关键词：radial glia ; GFAP ; vimentin ; migration ; adult neurogenesis ; teleost fish
• 刊名：Neuroscience
• 出版年：2012
• 期刊代码：50_03064522
• 类别：neu
• 出版时间：17 May, 2012
• 卷：210
• 期：Complete
• 页码：416-430
• 文件大小：6276 K

摘要

In contrast to mammals, in teleost fish radial glia persist beyond early development. This persistence parallels the enormous potential of teleosts to continuously generate a large number of new neurons in dozens of specific proliferation zones in the adult brain. In the present study, we characterized in the teleost fish Apteronotus leptorhynchus the immunological properties of radial glia in the corpus cerebelli鈥攁 cerebellar subdivision with particularly high proliferative activity鈥攁nd examined their possible function in the guidance of migrating young neurons. Radial glia stained immunopositive for glial fibrillary acidic protein (GFAP) and vimentin, and in most cases the two intermediate filament proteins co-localized. GFAP immunolabeling combined with immunohistochemistry against the mitotic marker 5-bromo-2'-deoxyuridine (BrdU) revealed an abundance of elongated BrdU-labeled nuclei closely apposed to, or localized within, GFAP-immunoreactive radial glia. The association of BrdU-labeled nuclei and GFAP-immunoreactive radial glial fibers was particularly pronounced 2 days after BrdU administration, when the migratory activity of the young cells is highest. When the new cells reach the granular layer, they start expressing the neuronal marker protein Hu C/D, but continue their close association with radial glial fibers. These results suggest the role of radial glia in the guidance of migrating adult-born neurons in the teleostean cerebellum. This function appears to be mediated both by somal translocation and by a glial-guided mode of locomotion.