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• 作者：Outi Nylander-Koski ; Harri Mustonen ; Tuula Kiviluoto and Eero Kivilaakso
• 关键词：cell volume regulation ; gastric mucosa ; microelectrodes ; Na+/K+ ATPase ; Na+&#8211 ; K+&#8211 ; 2Cl− ; cotransporter ; Na+/H+ exchanger
• 刊名：Digestive Diseases and Sciences
• 出版时间：November 2005
• 出版年：2005
• 期刊代码：5_01632116
• 类别：bus
• 卷：50
• 期：11
• 页码：2043-2049
• 数据来源：sp

摘要

Cell volume regulation was investigated in gastric surface epithelial cells during hypertonic conditions. Isolated Necturus antral mucosa was perfused on the serosal side with Ringer's solution (pH 7.25, 95%O₂/5%CO₂) and on the mucosal side successively with 150–500 mM NaCl. Amiloride, ouabain, and bumetanide were used to experimentally inhibit Na+/H+, Na+/K+ ATPase or Na+–K+–2Cl– ion transporters. Intracellular sodium activity and cell volume changes were measured with liquid sensor microelectrodes. The increase in intracellular sodium activity caused by luminal hyperosmolar exposure was mainly due to cell shrinkage. Inhibition of Na+/K+ ATPase or Na+–K+–2Cl– cotransporter increased hyperosmotic cell shrinkage (−52 卤 5%, −85 卤 19%, and −77 卤 9%for control, ouabain, and bumetanide, respectively). Inhibition of Na+/K+ ATPase increased intracellular sodium activity (from 18 卤 4 to 52 卤 12 mM). Cell volume regulation in gastric epithelial surface cells during mucosal hyperosmolar exposure is maintained by the basolateral Na+–K+–2Cl– cotransporter, while Na+/K+ ATPase maintains sodium balance, but Na+/H+ antiport seems to have a less important role.