Human Serum Protein Adsorption onto Synthesis Nano-Hydroxyapatite

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• 作者：M. Mohsen-Nia (12) moh.moh@cheme.caltech.edu
  M. Massah Bidgoli (1)
  M. Behrashi (3)
  A. Mohsen Nia (4)
• 关键词：Adsorption isotherm &#8211 ; Albumin &#8211 ; Human serum protein &#8211 ; UV&#8211 ; Visible &#8211 ; Nano ; hydroxyapatite
• 刊名：The Protein Journal
• 出版时间：February 2012
• 出版年：2012
• 期刊代码：221_1572-3887
• 类别：bio
• 卷：31
• 期：2
• 页码：150-157
• 数据来源：sp

摘要

Adsorption of human serum proteins (Albumin and total protein) onto high purity synthesis nano-hydroxyapatite (HA), Ca₁₀(PO₄)₆(OH)₂, has been studied in a wide temperature range by UV–visible spectrophotometer. Adsorption isotherm is basically important to describe how solutes interact with adsorbent, and is critical in optimizing the use of adsorbent. In the present study, the experimental results were fitted to the Langmuir, Freundlich, Temkin and Dubinin-Radushkevich (DR) models to obtain the characteristic parameters of each model and square of the correlation coefficients (R2). According to the results, the DR isotherm model had the best agreement with the experimental data. The effect of temperature on adsorption of human serum proteins (HSP) onto the synthesized nano-HA was studied. The experimental results indicated that temperature increase generally causes an increase in the adsorption of HSP onto the nano-HA. This is basically due to the effect of temperature on the HSP activity and its diffusion rate on HA surfaces.