The special features of protein adsorption isotherms on silica adsorbents
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- 作者：E. S. Chukhrai (1) Chukhrai@phys.chem.msu.ru
L. F. Atyaksheva (1)
O. S. Pilipenko (1) - 关键词：adsorption isotherm – ; protein – ; silica adsorbents – ; equilibrium constants – ; silochrome
- 刊名：Russian Journal of Physical Chemistry A ; Focus on Chemistry
- 出版时间：May 2011
- 出版年：2011
- 期刊代码：pc384_0036-0244
- 类别：ch
- 卷：85
- 期：5
- 页码：890-896
- 数据来源：sp
摘要
The adsorption isotherms of hemoglobin, peroxidase, and β-galactosidase on silochrome and mesoporous and biporous silicas were comparatively studied. Adsorption developed in two stages, including fast “reversible” protein adsorption (equilibrium was reached in t ≤ 1–2 h) and a “slow stage” of irreversible binding in t ≫ 24 h (multipoint adsorption). The corresponding equilibrium constants were determined. The mechanism of unlimited linear association of peroxidase in the adsorption layer on the surface of silochrome was established.