Congruent patterns of genetic variation in a burrowing freshwater crab revealed by allozymes and mt DNA sequence analysis
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- 作者：Savel R. Daniels ; Barbara A. Stewart and Peter A. Cook
- 关键词：allozymes ; sequencing ; freshwater crabs
- 刊名：Hydrobiologia
- 出版时间：2002
- 出版年：2002
- 期刊代码：7_00188158
- 类别：et
- 卷：468
- 期：1/3
- 页码：171-179
- 数据来源：sp
摘要
Five populations of the burrowing freshwater crab, Potamonautes calcaratus representing a total of 100 specimens, were collected from the Kruger National Park, South Africa. The population genetic structure of this species was investigated using both nuclear genetic markers (allozymes), and direct sequencing of a 610 base pair fragment the cytochrome oxidase 1(CO 1) subunit of the mitochondrial DNA. Electrophoresis of 21 allozyme loci revealed that moderate levels of genetic differentiation (F(ST) = 0.12) was present among populations. Sequence data for 20 individuals revealed the presence of ten haplotypes, the distribution of which showed no geographic structuring. The CT of 0.43 corroborates a moderate degree of genetic structuring. The nucleotide diversity () was low and ranged from 0.00 to 0.007. Sequence divergence amongst populations ranged from 0.49%to 1.47%. Both genetic markers revealed moderate population structuring, supporting the conclusion that populations share a common recent ancestry, with moderate levels of recent gene flow. These results provide evidence that allozyme and sequencing data may be congruent and that these independent markers can detect similar patterns of genetic differentiation. Results are discussed in light of contemporary factors that have been likely in sculpting the genetic structure.