A new microtiter plate-based screening method for microorganisms producing Alpha-amylase inhibitors
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- 作者：Zhi-Hua Feng (1)
Yuan-Shan Wang (1)
Yu-Guo Zheng (1) zhengyg@zjut.edu.cn - 关键词：α ; amylase inhibitors – ; human salivary amylase – ; 2 ; Chloro ; 4 ; nitrophenyl ; 4 ; O ; β ; D ; galactopyrano ; syl ; maltoside – ; diabetes mellitus – ; acarbose
- 刊名：Biotechnology and Bioprocess Engineering
- 出版时间：October 2011
- 出版年：2011
- 期刊代码：pc319_1226-8372
- 类别：ch
- 卷：16
- 期：5
- 页码：894-900
- 数据来源：sp
摘要
Alpha-amylase inhibitors are widely used by the pharmaceutical and agricultural industries, such as the treatment of diabetes and obesity and insect controller. Here, we developed a colorimetric method to screen for α-amylase inhibitor producing strains or mutants with higher α-amylase inhibitor productivity. This method relies on absorbance changes at 402 nm that are due to the inhibition of α-amylase catalyzed hydrolysis of 2-Chloro-4-nitrophenyl-4-O-β-D-galactopyranosyl-maltoside by α-amylase inhibitors. The assay can be performed on a microtiter plate, making it simple and convenient. Using this method, α-amylase inhibitor producing strains and mutants with higher α-amylase inhibitor productivity can be rapidly screened. One strain, ZJB-08196, with the highest α-amylase inhibition was isolated and identified as Actinoplanes utahensis, and one mutant with higher acarbose production was obtained by screening 3,000 variants using this method.