Optimization, production, and partial characterization of an alkalophilic amylase produced by sponge associated marine bacterium Halobacterium salinarum MMD047
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- 作者：S. Shanmughapriya (1)
G. Seghal Kiran (2)
Joseph Selvin (1) selvinj@rediffmail.com
R. Gandhimathi (1)
T. Bastin Baskar (1)
Aseer Manilal (1)
S. Sujith (1) - 关键词：amylase characterization ; extracellular amylase ; optimization ; marine amylase ; sponge bacteria
- 刊名：Biotechnology and Bioprocess Engineering
- 出版时间：February 2009
- 出版年：2009
- 期刊代码：pc319_1226-8372
- 类别：ch
- 卷：14
- 期：1
- 页码：67-75
- 数据来源：sp
摘要
An endosymbiont Halobacterium salinarum MMD047, which could produce high yields of amylase, was isolated from marine sponge Fasciospongia cavernosa, collected from the peninsular coast of India. Maximum production of enzyme was obtained in minimal medium supplemented with 1%sucrose. The enzyme was found to be produced constitutively even in the absence of starch. The optimum temperature and pH for the enzyme production was 40掳C and 8.0, respectively. The enzyme exhibited maximum activity in pH range of 6∼10 with an optimum pH of 9.0. The enzyme was stable at 40掳C and the enzyme activity decreased dramatically above 50掳C. Based on the present findings, the enzyme was characterized as relatively heat sensitive and alkalophilic amylase which can be developed for extensive industrial applications.